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Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)
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JoVE Journal Immunologia e infezioni
Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

DOI:
10.3791/53265-v

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06:20 min

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December 24, 2015

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, , , ,
1Regenerative Medicine Research Group, Institute of Cellular & System Medicine,National Health Research Institutes (NHRI), 2National Institute of Cancer Research,National Health Research Institutes (NHRI), 3Institute of Microbiology & Immunology,National Defense Medical Center

Capitoli

  • 00:05Titolo
  • 00:44Peripheral Blood Mononuclear Cell (PBMC) Isolation
  • 02:23MSC: Leukocyte Co-culture and Leukocyte Activation
  • 03:31Variation: Effector Suppression Assay
  • 04:38Results: Representative Suppressive Effects of MSC on Leukocyte Proliferation
  • 05:51Conclusion

Summary

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The immunomodulatory properties of human mesenchymal stem cells (MSC) appear increasingly relevant for clinical application. Using a co-culture system of MSCs and peripheral blood leukocytes pre-stained with the fluorescent dye carboxyfluorescein succinimidyl ester (CFSE), we describe the in vitro assessment of MSC immunomodulation on effector leukocyte proliferation and specific subpopulations.

Tags

Mesenchymal Stem CellsImmunomodulationLeukocyte ProliferationCFSEFlow CytometryEffector CellsImmune-related Diseases

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