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Experimental Demyelination and Remyelination of Murine Spinal Cord by Focal Injection of Lysolecithin
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Nörobilim
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JoVE Journal Nörobilim
Experimental Demyelination and Remyelination of Murine Spinal Cord by Focal Injection of Lysolecithin

Experimental Demyelination and Remyelination of Murine Spinal Cord by Focal Injection of Lysolecithin

DOI:
10.3791/52679-v

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08:57 min

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March 26, 2015

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, ,
1Department of Clinical Neurosciences,Hotchkiss Brain Institute at University of Calgary, 2Department of Oncology,Hotchkiss Brain Institute at University of Calgary

Bölümler

  • 00:05Başlık
  • 00:51Preparing the Injection Syringe
  • 02:55Preparing the Animal
  • 03:41Exposing the Spinal Cord
  • 05:36Injecting the Spinal Cord
  • 07:30Lysolecithin-induced Demyelination
  • 08:34Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Demyelinating diseases can be modeled in animals by focal application of lysolecithin into the CNS. A single injection of lysolecithin into mouse spinal cord produces a lesion that spontaneously repairs over time. The goal is to study factors involved in de- and remyelination, and to test agents for enhancing repair.

Etiketler

Experimental DemyelinationRemyelinationMurine Spinal CordLysolecithinMultiple SclerosisOligodendrocyte Precursor CellsAnimal ModelCentral Nervous SystemSpinal Cord White MatterRemyelination-promoting Therapeutics

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