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Validación de nanocuerpo y basados ​​en anticuerpos<em> En Vivo</em> Experimentos tumor de xenoinjerto NIRF-de formación de imágenes en ratones utilizando<em> Ex Vivo</em> Citometría de flujo y microscopía
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Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

Validación de nanocuerpo y basados ​​en anticuerpos<em> En Vivo</em> Experimentos tumor de xenoinjerto NIRF-de formación de imágenes en ratones utilizando<em> Ex Vivo</em> Citometría de flujo y microscopía

DOI:

08:09 min

April 06, 2015

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Bölümler

  • 00:05Başlık
  • 02:11In Vivo Imaging
  • 03:07Harvesting and Preparation of Tumors
  • 04:19FACS Analysis
  • 06:01Results: Nanobody and mAb Staining of Tumors
  • 07:09Conclusion

Özet

Otomatik Çeviri

This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence xenograft imaging experiments in mice using fluorophore labelled nanobodies and conventional antibodies.

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