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Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
JoVE Journal
医学
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JoVE Journal 医学
Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

DOI:
10.3791/52462-v

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08:09 min

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April 06, 2015

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, , , , , , , , , ,
1Department of Diagnostic and Interventional Radiology,University Medical Center, Hamburg, 2Institute of Immunology,University Medical Center, Hamburg, 3University Cancer Center Hamburg,University Medical Center, Hamburg, 4Department of Oncology and Hematology,University Medical Center, Hamburg

章

  • 00:05標題
  • 02:11In Vivo Imaging
  • 03:07Harvesting and Preparation of Tumors
  • 04:19FACS Analysis
  • 06:01Results: Nanobody and mAb Staining of Tumors
  • 07:09Conclusion

概要

自動翻訳

自動翻訳

This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence xenograft imaging experiments in mice using fluorophore labelled nanobodies and conventional antibodies.

タグ

NanobodyAntibodyIn VivoTumor XenograftNIRF ImagingFlow CytometryMicroscopySubcutaneous TumorAntigen-negativeAntigen-positiveAlexaFluor680Non-invasive ImagingEx Vivo Validation

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