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Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
JoVE Journal
Medizin
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JoVE Journal Medizin
Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

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08:09 min

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April 06, 2015

DOI:

10.3791/52462-v

DOI:
10.3791/52462-v

•

08:09 min
April 06, 2015

•

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1Department of Diagnostic and Interventional Radiology,University Medical Center, Hamburg, 2Institute of Immunology,University Medical Center, Hamburg, 3University Cancer Center Hamburg,University Medical Center, Hamburg, 4Department of Oncology and Hematology,University Medical Center, Hamburg

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This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence xenograft imaging experiments in mice using fluorophore labelled nanobodies and conventional antibodies.

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NanobodyAntibodyIn VivoTumor XenograftNIRF ImagingFlow CytometryMicroscopySubcutaneous TumorAntigen-negativeAntigen-positiveAlexaFluor680Non-invasive ImagingEx Vivo Validation

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Bannas, P., Lenz, A., Kunick, V., Fumey, W., Rissiek, B., Schmid, J., Haag, F., Leingärtner, A., Trepel, M., Adam, G., Koch-Nolte, F. Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy. J. Vis. Exp. (98), e52462, doi:10.3791/52462 (2015).

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