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原代鼠脑微血管内皮细胞的分离
JoVE Journal
Nörobilim
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JoVE Journal Nörobilim
Isolation of Primary Murine Brain Microvascular Endothelial Cells
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原代鼠脑微血管内皮细胞的分离

DOI:
10.3791/52204-v

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08:14 min

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November 14, 2014

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, , , ,
1Department of Neurology,University of Münster, 2Interdisciplinary Center for Clinical Research (IZKF) Münster, 3Institute of Physiology I — Neuropathophysiology I,University of Münster

Bölümler

  • 00:05Başlık
  • 01:46Isolation of Murine Brain Microvascular Endothelial Cells (BMEC)
  • 05:41Results: Morphological and Functional Properties of Murine BMECs
  • 07:21Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Brain microvascular endothelial cells (BMEC) are interconnected by specific junctional proteins forming a highly regulated barrier separating blood and the central nervous system (CNS), the so-called blood-brain-barrier (BBB). The isolation of primary murine brain microvascular endothelial cells, as discussed in this protocol, enables detailed in vitro studies of the BBB.

Etiketler

Primary Murine Brain Microvascular Endothelial CellsBlood-brain-barrierNeurovascular UnitIn Vitro ModelIsolationPurificationCulture

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