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Isolation of Primary Murine Brain Microvascular Endothelial Cells
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Isolation of Primary Murine Brain Microvascular Endothelial Cells

Isolation of Primary Murine Brain Microvascular Endothelial Cells

DOI:
10.3791/52204-v

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08:14 min

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November 14, 2014

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, , , ,
1Department of Neurology,University of Münster, 2Interdisciplinary Center for Clinical Research (IZKF) Münster, 3Institute of Physiology I — Neuropathophysiology I,University of Münster

Chapters

  • 00:05Title
  • 01:46Isolation of Murine Brain Microvascular Endothelial Cells (BMEC)
  • 05:41Results: Morphological and Functional Properties of Murine BMECs
  • 07:21Conclusion

Summary

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Brain microvascular endothelial cells (BMEC) are interconnected by specific junctional proteins forming a highly regulated barrier separating blood and the central nervous system (CNS), the so-called blood-brain-barrier (BBB). The isolation of primary murine brain microvascular endothelial cells, as discussed in this protocol, enables detailed in vitro studies of the BBB.

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Primary Murine Brain Microvascular Endothelial CellsBlood-brain-barrierNeurovascular UnitIn Vitro ModelIsolationPurificationCulture

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