Dergi
/
Nörobilim
/
בידוד של תאים הראשוניים Murine מוח כלי הדם האנדותל
JoVE Journal
Nörobilim
This content is Free Access.
JoVE Journal Nörobilim
Isolation of Primary Murine Brain Microvascular Endothelial Cells
Please note that all translations are automatically generated. Click here for the English version.

בידוד של תאים הראשוניים Murine מוח כלי הדם האנדותל

31,410 Views

•

08:14 min

•

November 14, 2014

DOI:

10.3791/52204-v

DOI:
10.3791/52204-v

•

08:14 min
November 14, 2014

•

67 Views
, , , ,
1Department of Neurology,University of Münster, 2Interdisciplinary Center for Clinical Research (IZKF) Münster, 3Institute of Physiology I — Neuropathophysiology I,University of Münster

Özet

Automatically generated

Brain microvascular endothelial cells (BMEC) are interconnected by specific junctional proteins forming a highly regulated barrier separating blood and the central nervous system (CNS), the so-called blood-brain-barrier (BBB). The isolation of primary murine brain microvascular endothelial cells, as discussed in this protocol, enables detailed in vitro studies of the BBB.

Explore More Videos

Primary Murine Brain Microvascular Endothelial CellsBlood-brain-barrierNeurovascular UnitIn Vitro ModelIsolationPurificationCulture

Read Article

Cite this Article

Ruck, T., Bittner, S., Epping, L., Herrmann, A. M., Meuth, S. G. Isolation of Primary Murine Brain Microvascular Endothelial Cells. J. Vis. Exp. (93), e52204, doi:10.3791/52204 (2014).

Download .ris file

Copy

Share Video

.

Copy