Dergi
/
İmmünoloji ve Enfeksiyon
/
Metodologia per la generazione efficiente di proteine del virus vaccinia taggate fluorescentmente
JoVE Journal
İmmünoloji ve Enfeksiyon
This content is Free Access.
JoVE Journal İmmünoloji ve Enfeksiyon
Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
Please note that all translations are automatically generated. Click here for the English version.

Metodologia per la generazione efficiente di proteine del virus vaccinia taggate fluorescentmente

7,309 Views

•

09:27 min

•

January 17, 2014

DOI:

10.3791/51151-v

DOI:
10.3791/51151-v

•

09:27 min
January 17, 2014

•

2 Views
, , , , ,
1School of Molecular Bioscience,University of Sydney, 2Tıp Fakültesi,Center for Vascular Research, 3Asia Pacific Centre for Animal Health, Faculty of Veterinary Science,University of Melbourne

Özet

Automatically generated

Qui viene descritto un protocollo rapido e modulare per la generazione di virus vaccinia ricombinanti che esprimono simultaneamente proteine contrassegnate fluorescentmente utilizzando il metodo della selezione dominante transitoria.

Explore More Videos

Fluorescent Protein TaggingVaccinia VirusRecombinant Virus GenerationHomologous RecombinationTransient Dominant SelectionMetabolic SelectionLive-cell MicroscopyVirus-host Interactions

Read Article

Cite this Article

Marzook, N. B., Procter, D. J., Lynn, H., Yamamoto, Y., Horsington, J., Newsome, T. P. Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins. J. Vis. Exp. (83), e51151, doi:10.3791/51151 (2014).

Download .ris file

Copy

Share Video

.

Copy