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Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen
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Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen
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March 21, 2014
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Özet
Automatically generated
The wormsorter facilitates genetic screens in Caenorhabditis elegans by sorting worms according to expression of fluorescent reporters. Here, we describe a new usage: sorting according to colonization by a GFP-expressing pathogen, and we employ it to examine the poorly understood role of pathogen recognition in initiating immune responses.
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Twumasi-Boateng, K., Berg, M., Shapira, M. Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen. J. Vis. Exp. (85), e51090, doi:10.3791/51090 (2014).
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