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Immunology and Infection

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JoVE Journal Immunology and Infection
Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen
 

Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen

Article DOI: 10.3791/51090-v • 11:18 min • March 21st, 2014
March 21st, 2014 •
1, 1, 1
1Department of Integrative Biology, University of California, Berkeley

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The wormsorter facilitates genetic screens in Caenorhabditis elegans by sorting worms according to expression of fluorescent reporters. Here, we describe a new usage: sorting according to colonization by a GFP-expressing pathogen, and we employ it to examine the poorly understood role of pathogen recognition in initiating immune responses.

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Keywords: C. Elegans Caenorhabditis Elegans Bacterial Pathogen Wormsorter FACS GFP Pseudomonas Aeruginosa Immune Response Pathogen Colonization Pathogen-associated Molecular Patterns Damage Detection
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