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Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen

Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen

8,257 Views

11:18 min

March 21, 2014

DOI:

11:18 min
March 21, 2014

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Summary

Automatically generated

The wormsorter facilitates genetic screens in Caenorhabditis elegans by sorting worms according to expression of fluorescent reporters. Here, we describe a new usage: sorting according to colonization by a GFP-expressing pathogen, and we employ it to examine the poorly understood role of pathogen recognition in initiating immune responses.

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