Dergi
/
Nörobilim
/
Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis

Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis

12,294 Views

•

09:38 min

•

August 30, 2013

DOI:

10.3791/50769-v

DOI:
10.3791/50769-v

•

09:38 min
August 30, 2013

•

1 Views
, , , , ,
1Department of Neurologic Surgery,Mayo Clinic College of Medicine, 2Department of Anatomy,Korea University College of Medicine, 3Biyokimya ve Moleküler Biyoloji Anabilim Dalı,Mayo Clinic College of Medicine

Özet

Automatically generated

During adult hippocampal neurogenesis, a distinct set of genetic markers are expressed when a quiescent neural stem cell sequentially progresses and develops into a functionally integrated neuron in the circuit. Using heat-induced antigen retrieval, progenitor cell types that are otherwise difficult to detect are identified with improved effectiveness.

Explore More Videos

Heat-induced Antigen RetrievalImmunohistochemistryAdult Hippocampal NeurogenesisNeural Stem CellProgenitor Cell TypesProliferationDifferentiationMaturationFunctional IntegrationPathophysiology

Read Article

Cite this Article

Hussaini, S. M., Jun, H., Cho, C. H., Kim, H. J., Kim, W. R., Jang, M. Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis. J. Vis. Exp. (78), e50769, doi:10.3791/50769 (2013).

Download .ris file

Copy

Share Video

.

Copy