During adult hippocampal neurogenesis, a distinct set of genetic markers are expressed when a quiescent neural stem cell sequentially progresses and develops into a functionally integrated neuron in the circuit. Using heat-induced antigen retrieval, progenitor cell types that are otherwise difficult to detect are identified with improved effectiveness.
Hussaini, S. M., Jun, H., Cho, C. H., Kim, H. J., Kim, W. R., Jang, M. Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis. J. Vis. Exp. (78), e50769, doi:10.3791/50769 (2013).