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Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis
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神经科学
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JoVE 杂志 神经科学
Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis

Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis

DOI:
10.3791/50769-v

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09:38 min

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August 30, 2013

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, , , , ,
1Department of Neurologic Surgery,Mayo Clinic College of Medicine, 2Department of Anatomy,Korea University College of Medicine, 3Department of Biochemistry and Molecular Biology,Mayo Clinic College of Medicine

Chapters

  • 00:05Title
  • 02:23Pre-immunohistochemistry
  • 03:44Heat-induced Antigen Retrieval
  • 04:52Primary and Secondary Antibody Staining
  • 06:50Results: Development of Neural Stem Cell During Adult Hippocampal Neurogenesis
  • 08:27Conclusion

Summary

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自动翻译

During adult hippocampal neurogenesis, a distinct set of genetic markers are expressed when a quiescent neural stem cell sequentially progresses and develops into a functionally integrated neuron in the circuit. Using heat-induced antigen retrieval, progenitor cell types that are otherwise difficult to detect are identified with improved effectiveness.

Tags

Heat-induced Antigen RetrievalImmunohistochemistryAdult Hippocampal NeurogenesisNeural Stem CellProgenitor Cell TypesProliferationDifferentiationMaturationFunctional IntegrationPathophysiology

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