/
/
Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish
Bölümler
- 00:05Başlık
- 01:02Zebrafish Care and Maintenance
- 01:59Microplate Reader Programming
- 03:57Fluorescence Quantification
- 04:34Data Analysis
- 05:41Results: Respiratory Burst Response in Zebrafish Embryos
- 06:41Conclusion
The innate immune response protects organisms against pathogen infection. A critical component of the innate immune response, the phagocyte respiratory burst, generates reactive oxygen species that kill invading microorganisms. We describe a respiratory burst assay that quantifies reactive oxygen species produced when the innate immune response is chemically induced.
