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强大的3D直接标记的探针DNA鱼
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
Robust 3D DNA FISH Using Directly Labeled Probes
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强大的3D直接标记的探针DNA鱼

DOI:
10.3791/50587-v

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12:16 min

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August 15, 2013

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, , , ,
1Nuclear Dynamics Programme,The Babraham Institute, 2Epigenetics Programme,The Babraham Institute, 3Centre for Trophoblast Research,University of Cambridge

Bölümler

  • 00:05Başlık
  • 01:18Generating Directly Labeled DNA Probes by Nick Translation
  • 02:21Day 1: Probe Precipitation
  • 04:04Day 1: Treatment of the Cells
  • 08:03Day 2: Final Washes and Mounting
  • 09:24FISH Probe Quality Control and Application
  • 11:33Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

我们描述一个强大的和灵活的三维DNA鱼直接标记的荧光探针分析核架构协议。

Etiketler

3D DNA FISHDirectly Labeled ProbesNick-translationFreeze-thaw PermeabilizationNuclear OrganizationChromosomal RegionsHigh-throughput Imaging

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