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Robust 3D DNA FISH Using Directly Labeled Probes
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Biologie
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JoVE Journal Biologie
Robust 3D DNA FISH Using Directly Labeled Probes

Robust 3D DNA FISH Using Directly Labeled Probes

DOI:
10.3791/50587-v

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12:16 min

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August 15, 2013

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, , , ,
1Nuclear Dynamics Programme,The Babraham Institute, 2Epigenetics Programme,The Babraham Institute, 3Centre for Trophoblast Research,University of Cambridge

Kapitel

  • 00:05Titel
  • 01:18Generating Directly Labeled DNA Probes by Nick Translation
  • 02:21Day 1: Probe Precipitation
  • 04:04Day 1: Treatment of the Cells
  • 08:03Day 2: Final Washes and Mounting
  • 09:24FISH Probe Quality Control and Application
  • 11:33Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We describe a robust and versatile protocol for analyzing nuclear architecture by 3D DNA FISH using directly labeled fluorescent probes.

Tags

3D DNA FISHDirectly Labeled ProbesNick-translationFreeze-thaw PermeabilizationNuclear OrganizationChromosomal RegionsHigh-throughput Imaging

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