ジャーナル
/
生物学
/
Robust 3D DNA FISH Using Directly Labeled Probes
JoVE Journal
生物学
This content is Free Access.
JoVE Journal 生物学
Robust 3D DNA FISH Using Directly Labeled Probes

Robust 3D DNA FISH Using Directly Labeled Probes

34,053 Views

•

12:16 min

•

August 15, 2013

DOI:

10.3791/50587-v

DOI:
10.3791/50587-v

•

12:16 min
August 15, 2013

•

6 Views
, , , ,
1Nuclear Dynamics Programme,The Babraham Institute, 2Epigenetics Programme,The Babraham Institute, 3Centre for Trophoblast Research,University of Cambridge

概要

Automatically generated

We describe a robust and versatile protocol for analyzing nuclear architecture by 3D DNA FISH using directly labeled fluorescent probes.

Explore More Videos

3D DNA FISHDirectly Labeled ProbesNick-translationFreeze-thaw PermeabilizationNuclear OrganizationChromosomal RegionsHigh-throughput Imaging

Read Article

Cite this Article

Bolland, D. J., King, M. R., Reik, W., Corcoran, A. E., Krueger, C. Robust 3D DNA FISH Using Directly Labeled Probes. J. Vis. Exp. (78), e50587, doi:10.3791/50587 (2013).

Download .ris file

Copy

Share Video

.

Copy