ジャーナル
/
生物学
/
Robust 3D DNA FISH Using Directly Labeled Probes
JoVE Journal
生物学
This content is Free Access.
JoVE Journal 生物学
Robust 3D DNA FISH Using Directly Labeled Probes

Robust 3D DNA FISH Using Directly Labeled Probes

DOI:
10.3791/50587-v

•

12:16 min

•

August 15, 2013

•

, , , ,
1Nuclear Dynamics Programme,The Babraham Institute, 2Epigenetics Programme,The Babraham Institute, 3Centre for Trophoblast Research,University of Cambridge

章

  • 00:05標題
  • 01:18Generating Directly Labeled DNA Probes by Nick Translation
  • 02:21Day 1: Probe Precipitation
  • 04:04Day 1: Treatment of the Cells
  • 08:03Day 2: Final Washes and Mounting
  • 09:24FISH Probe Quality Control and Application
  • 11:33Conclusion

概要

自動翻訳

自動翻訳

We describe a robust and versatile protocol for analyzing nuclear architecture by 3D DNA FISH using directly labeled fluorescent probes.

タグ

3D DNA FISHDirectly Labeled ProbesNick-translationFreeze-thaw PermeabilizationNuclear OrganizationChromosomal RegionsHigh-throughput Imaging

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

DNA-based Fish Species Identification Protocol

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Assessing Species-specific Contributions To Craniofacial Development Using Quail-duck Chimeras

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans

Fish Sperm Assessment Using Software and Cooling Devices

Read Article