Dergi
/
Nörobilim
/
Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

15,666 Views

•

11:48 min

•

July 24, 2013

DOI:

10.3791/50412-v

DOI:
10.3791/50412-v

•

11:48 min
July 24, 2013

•

1 Views
, , ,
1Institute of Anatomy and Cell Biology,University of Heidelberg , 2Institut de recherches cliniques de Montreal

Özet

Automatically generated

Despite the functional and medical importance of the hypothalamus, in utero genetic manipulation of its development has rarely been attempted. We show a detailed procedure for in utero electroporation into the mouse hypothalamus and show representative results of total and partial (regional) hypothalamic transfection.

Explore More Videos

Genetic ManipulationMouseDeveloping HypothalamusIn Utero ElectroporationGenetic ModificationMammalian BrainMouse MutantsDeveloping BrainCortexThalamusHypothalamusHypothalamic NeuroepitheliumMammillary BodyElectroporation

Read Article

Cite this Article

Haddad-Tóvolli, R., Szabó, N., Zhou, X., Alvarez-Bolado, G. Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation. J. Vis. Exp. (77), e50412, doi:10.3791/50412 (2013).

Download .ris file

Copy

Share Video

.

Copy