Experience-dependent molecular changes in neurons are essential for the brain's ability to adapt in response to behavioral challenges. An in vivo two-photon imaging method is described here that allows the tracking of such molecular changes in individual cortical neurons through genetically encoded reporters.
Cao, V. Y., Ye, Y., Mastwal, S. S., Lovinger, D. M., Costa, R. M., Wang, K. H. In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons. J. Vis. Exp. (71), e50148, doi:10.3791/50148 (2013).