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Hippocampal Insulin Microinjection and In vivo Microdialysis During Spatial Memory Testing
JoVE Journal
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JoVE Journal Nörobilim
Hippocampal Insulin Microinjection and In vivo Microdialysis During Spatial Memory Testing

Hippocampal Insulin Microinjection and In vivo Microdialysis During Spatial Memory Testing

DOI:
10.3791/4451-v

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10:32 min

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January 11, 2013

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, ,
1Behavioral Neuroscience,University at Albany

Bölümler

  • 00:05Başlık
  • 01:28Cannulation and Set-up
  • 04:29Microdialysis Set-up and Equilibration
  • 06:33Sample Collection during Behavioral Testing
  • 08:55Hippocampal Glucose Levels during Behavioral Testing
  • 09:49Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Modulation of hippocampally-dependent spatial working memory by direct intrahippocampal microinjection, accompanied and followed by in vivo microdialysis for metabolites in conscious, behaving animals.

Etiketler

Hippocampal Insulin MicroinjectionIn Vivo MicrodialysisSpatial Memory TestingGlucose MetabolismNeural Activity Marker2-deoxyglucoseFunctional Magnetic Resonance ImagingAnesthesiaBehavioral MeasuresBrain State AlterationContinuous MeasurementBrain Extracellular Fluid ConcentrationsLactateMetabolitesAwake AnimalsUnrestrained AnimalsTasks Designed For Specific Brain RegionsAcute Pharmacological ManipulationHippocampal MeasurementsSpatial Working Memory TaskSpontaneous AlternationEnhanced GlycolysisIntrahippocampal Insulin AdministrationMemory ImprovementHippocampal GlycolysisMicrodialysis Probe DeliveryMetabolite MeasurementSpontaneous Alternation TaskHippocampal Function Measure

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