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Hippocampal Insulin Microinjection and In vivo Microdialysis During Spatial Memory Testing
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Hippocampal Insulin Microinjection and In vivo Microdialysis During Spatial Memory Testing
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January 11, 2013
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Modulation of hippocampally-dependent spatial working memory by direct intrahippocampal microinjection, accompanied and followed by in vivo microdialysis for metabolites in conscious, behaving animals.
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Hippocampal Insulin MicroinjectionIn Vivo MicrodialysisSpatial Memory TestingGlucose MetabolismNeural Activity Marker2-deoxyglucoseFunctional Magnetic Resonance ImagingAnesthesiaBehavioral MeasuresBrain State AlterationContinuous MeasurementBrain Extracellular Fluid ConcentrationsLactateMetabolitesAwake AnimalsUnrestrained AnimalsTasks Designed For Specific Brain RegionsAcute Pharmacological ManipulationHippocampal MeasurementsSpatial Working Memory TaskSpontaneous AlternationEnhanced GlycolysisIntrahippocampal Insulin AdministrationMemory ImprovementHippocampal GlycolysisMicrodialysis Probe DeliveryMetabolite MeasurementSpontaneous Alternation TaskHippocampal Function Measure
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McNay, E. C., Sandusky, L. A., Pearson-Leary, J. Hippocampal Insulin Microinjection and In vivo Microdialysis During Spatial Memory Testing. J. Vis. Exp. (71), e4451, doi:10.3791/4451 (2013).
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