/
/
Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes
11,761 Views
•
13:42 min
•
March 08, 2013
DOI:
Özet
Automatically generated
The presented method offers a way to detect functional effective cardiotropic autoantibodies in the plasma of patients with dilated cardiomyopathy, irrespective of the specific antigen, by analysing the impact of isolated patient immunoglobulin on cellular shortening and intracellular calcium transients in isolated rat cardiomyocytes.
Explore More Videos
MeasurementAntibody EffectsCellular FunctionIsolated CardiomyocytesDilated CardiomyopathyHeart FailureGenetic DispositionToxic SubstancesAutoantibodiesCardiac EpitopesOnset And ProgressionDiseaseHemodynamic ImprovementImmunoadsorptionSpecific AntigensImmunoassaysStimulating AutoantibodiesBlocking AutoantibodiesCardiotropic AntibodiesFunctional RegionsCardiac Proteins
Read Article
Cite this Article
Eckerle, L. G., Felix, S. B., Herda, L. R. Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes. J. Vis. Exp. (73), e4237, doi:10.3791/4237 (2013).
Copy