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Imunologia e Infecção
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Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes

Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes

DOI:
10.3791/4237-v

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13:42 min

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March 08, 2013

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, ,
1Department of Internal Medicine B,University Medicine Greifswald

Capítulos

  • 00:05Título
  • 01:51IgG Isolation from Patient Samples
  • 04:39Isolation of Rat Cardiomyocytes
  • 08:27Fura-2 Staining of Cardiomyocytes
  • 09:43Recording of Cell Shortening and Ca2+ Transients
  • 12:24Results: The Measurement of an IgG Sample Containing Cardiodepressive Antibodies
  • 13:15Conclusion

Summary

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The presented method offers a way to detect functional effective cardiotropic autoantibodies in the plasma of patients with dilated cardiomyopathy, irrespective of the specific antigen, by analysing the impact of isolated patient immunoglobulin on cellular shortening and intracellular calcium transients in isolated rat cardiomyocytes.

Tags

MeasurementAntibody EffectsCellular FunctionIsolated CardiomyocytesDilated CardiomyopathyHeart FailureGenetic DispositionToxic SubstancesAutoantibodiesCardiac EpitopesOnset And ProgressionDiseaseHemodynamic ImprovementImmunoadsorptionSpecific AntigensImmunoassaysStimulating AutoantibodiesBlocking AutoantibodiesCardiotropic AntibodiesFunctional RegionsCardiac Proteins

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