/
/
Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes
11,763 Views
•
13:42 min
•
March 08, 2013
DOI:
Summary
Automatically generated
The presented method offers a way to detect functional effective cardiotropic autoantibodies in the plasma of patients with dilated cardiomyopathy, irrespective of the specific antigen, by analysing the impact of isolated patient immunoglobulin on cellular shortening and intracellular calcium transients in isolated rat cardiomyocytes.
Explore More Videos
MeasurementAntibody EffectsCellular FunctionIsolated CardiomyocytesDilated CardiomyopathyHeart FailureGenetic DispositionToxic SubstancesAutoantibodiesCardiac EpitopesOnset And ProgressionDiseaseHemodynamic ImprovementImmunoadsorptionSpecific AntigensImmunoassaysStimulating AutoantibodiesBlocking AutoantibodiesCardiotropic AntibodiesFunctional RegionsCardiac Proteins
Read Article
Cite this Article
Eckerle, L. G., Felix, S. B., Herda, L. R. Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes. J. Vis. Exp. (73), e4237, doi:10.3791/4237 (2013).
Copy