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利用同位素标记的质谱剖析巯基氧化还原蛋白质
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
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利用同位素标记的质谱剖析巯基氧化还原蛋白质

15,829 Views

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12:07 min

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March 24, 2012

DOI:

10.3791/3766-v

DOI:
10.3791/3766-v

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12:07 min
March 24, 2012

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1 Views
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1Plant Molecular and Cellular Biology Program,Florida Üniversitesi , 2Biyoloji Bölümü,Florida Üniversitesi , 3Interdisciplinary Center for Biotechnology Research,Florida Üniversitesi , 4Genetics Institute,Florida Üniversitesi

Etiketler

Thiol Redox ProteomeIsotope Tagging Mass SpectrometryPseudomonas Syringae Pv. TomatoBacterial Speck DiseaseSolanum LycopersicumBrassica SpeciesArabidopsis ThalianaReactive Oxygen Species (ROS)Hydrogen PeroxideDAB StainingRedox Status Of ProteinsCysteine OxidationTandem Mass Tag (TMT) ReagentsCysteine-reactive TMT (cysTMT) Tags

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Cite this Article

Parker, J., Zhu, N., Zhu, M., Chen, S. Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry. J. Vis. Exp. (61), e3766, doi:10.3791/3766 (2012).

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