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Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow
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Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow
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January 14, 2012
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We show the method for performing intravital microscopy of the spleen using GFP transgenic malaria parasites and the quantification of parasite mobility and blood flow within this organ.
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Intravital MicroscopySpleenQuantitative AnalysisParasite MobilityBlood FlowMalaria ModelsParasite-host InteractionsPre-erythrocytic StagesSkin Lymph NodesHepatocyte-derived MerosomeMerozoitesErythrocytesProtein ExportPlasmodium DevelopmentDynamic PassageLymphoid OrganInfected Red Blood CellsPlasmodium YoeliiBalb/c MiceBarrier CellsFibroblastic OriginRed Pulp
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Ferrer, M., Martin-Jaular, L., Calvo, M., del Portillo, H. A. Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow. J. Vis. Exp. (59), e3609, doi:10.3791/3609 (2012).
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