Journal
/
Immunologie et infection
/
Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow
Journal JoVE
Immunologie et infection
Author Produced
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

17,986 Views

•

11:36 min

•

January 14, 2012

DOI:

10.3791/3609-v

DOI:
10.3791/3609-v

•

11:36 min
January 14, 2012

•

3 Views
, , ,
1Department of poverty related diseases,Barcelona Centre for International Health Research, 2Confocal Microscopy Unit,University of Barcelona- Scientific and Technological Centers, 3Institució Catalana de Recerca i Estudis Avançats (ICREA)

Summary

Automatically generated

We show the method for performing intravital microscopy of the spleen using GFP transgenic malaria parasites and the quantification of parasite mobility and blood flow within this organ.

Explore More Videos

Intravital MicroscopySpleenQuantitative AnalysisParasite MobilityBlood FlowMalaria ModelsParasite-host InteractionsPre-erythrocytic StagesSkin Lymph NodesHepatocyte-derived MerosomeMerozoitesErythrocytesProtein ExportPlasmodium DevelopmentDynamic PassageLymphoid OrganInfected Red Blood CellsPlasmodium YoeliiBalb/c MiceBarrier CellsFibroblastic OriginRed Pulp

Read Article

Cite this Article

Ferrer, M., Martin-Jaular, L., Calvo, M., del Portillo, H. A. Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow. J. Vis. Exp. (59), e3609, doi:10.3791/3609 (2012).

Download .ris file

Copy

Share Video

.

Copy