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Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

17,986 Views

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11:36 min

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January 14, 2012

DOI:

10.3791/3609-v

DOI:
10.3791/3609-v

•

11:36 min
January 14, 2012

•

3 Views
, , ,
1Department of poverty related diseases,Barcelona Centre for International Health Research, 2Confocal Microscopy Unit,University of Barcelona- Scientific and Technological Centers, 3Institució Catalana de Recerca i Estudis Avançats (ICREA)

Summary

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We show the method for performing intravital microscopy of the spleen using GFP transgenic malaria parasites and the quantification of parasite mobility and blood flow within this organ.

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Intravital MicroscopySpleenQuantitative AnalysisParasite MobilityBlood FlowMalaria ModelsParasite-host InteractionsPre-erythrocytic StagesSkin Lymph NodesHepatocyte-derived MerosomeMerozoitesErythrocytesProtein ExportPlasmodium DevelopmentDynamic PassageLymphoid OrganInfected Red Blood CellsPlasmodium YoeliiBalb/c MiceBarrier CellsFibroblastic OriginRed Pulp

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Ferrer, M., Martin-Jaular, L., Calvo, M., del Portillo, H. A. Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow. J. Vis. Exp. (59), e3609, doi:10.3791/3609 (2012).

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