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Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells
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Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells

Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells

DOI:
10.3791/3159-v

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16:55 min

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October 26, 2011

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, , , ,
1Charles C. Gates Regenerative Medicine and Stem Cell Biology Program,University of Colorado Denver, 2Tıp Fakültesi,University of Colorado Denver, 3Cancer Center,University of Colorado Denver, 4Webb Waring Institute,University of Colorado Denver

Bölümler

  • 00:05Başlık
  • 01:49Lung Isolation
  • 04:05Preparation of a Single Cell Suspension from Lung Tissue
  • 07:15Flow Cytometry Analysis to Define and Isolate a Lung MSC Population using Hoechst dye Efflux to Detect a Side Population
  • 13:16Enumeration of Lung MSC using a Colony Forming Assay (CFU-F)
  • 14:49Representative Analysis of Isolated MSCs
  • 16:08Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

In this article we demonstrate the isolation of murine resident lung mesenchymal stem cells (lung MSC), their expansion, characterization and analysis of immunomodulatory properties.

Etiketler

IsolationCharacterizationHoechstlow CD45negativeMouse LungMesenchymal Stem CellsRegulatorsRegenerationFibrosisInflammationAngiogenesisTumor FormationInjuryDiseasesPulmonary FibrosisArterial HypertensionTechnologyDefine Set Of MarkersRepeated IsolationWell-characterized Stem Cell PopulationFlow CytometrySpecific Cell TypeFunction

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