Isolation &#38; Characterization of Hoechst<sup>low</sup> CD45<sup>negative</sup> Mouse Lung Mesenchymal Stem Cells

Kelsey S. Chow; DuHyun Jun; Karen M. Helm; David H. Wagner; Susan M. Majka

doi:10.3791/3159

ジャーナル

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生物学

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Isolation & Characterization of Hoechst^low CD45^negative Mouse Lung Mesenchymal Stem Cells

JoVE Journal
生物学

This content is Free Access.

JoVE Journal 生物学

Isolation & Characterization of Hoechst^low CD45^negative Mouse Lung Mesenchymal Stem Cells

20,138 Views

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16:55 min

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October 26, 2011

DOI:

10.3791/3159-v

DOI:

10.3791/3159-v

•

16:55 min

October 26, 2011

•

4 Views

Kelsey S. Chow^1,2, DuHyun Jun^1,2, Karen M. Helm³, David H. Wagner^1,2,4, Susan M. Majka^1,2,3

¹Charles C. Gates Regenerative Medicine and Stem Cell Biology Program,University of Colorado Denver, ²医学部,University of Colorado Denver, ³Cancer Center,University of Colorado Denver, ⁴Webb Waring Institute,University of Colorado Denver

概要

In this article we demonstrate the isolation of murine resident lung mesenchymal stem cells (lung MSC), their expansion, characterization and analysis of immunomodulatory properties.

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Isolation & Characterization of Hoechst^low CD45^negative Mouse Lung Mesenchymal Stem Cells

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Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells

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Isolation & Characterization of Hoechst^low CD45^negative Mouse Lung Mesenchymal Stem Cells