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Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells
JoVE 杂志
生物学
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JoVE 杂志 生物学
Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells

Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells

20,138 Views

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16:55 min

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October 26, 2011

DOI:

10.3791/3159-v

DOI:
10.3791/3159-v

•

16:55 min
October 26, 2011

•

4 Views
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1Charles C. Gates Regenerative Medicine and Stem Cell Biology Program,University of Colorado Denver, 2Department of Medicine,University of Colorado Denver, 3Cancer Center,University of Colorado Denver, 4Webb Waring Institute,University of Colorado Denver

Summary

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In this article we demonstrate the isolation of murine resident lung mesenchymal stem cells (lung MSC), their expansion, characterization and analysis of immunomodulatory properties.

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IsolationCharacterizationHoechstlow CD45negativeMouse LungMesenchymal Stem CellsRegulatorsRegenerationFibrosisInflammationAngiogenesisTumor FormationInjuryDiseasesPulmonary FibrosisArterial HypertensionTechnologyDefine Set Of MarkersRepeated IsolationWell-characterized Stem Cell PopulationFlow CytometrySpecific Cell TypeFunction

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Chow, K. S., Jun, D., Helm, K. M., Wagner, D. H., Majka, S. M. Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells. J. Vis. Exp. (56), e3159, doi:10.3791/3159 (2011).

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