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Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells

Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells

18,712 Views

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10:09 min

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January 03, 2012

DOI:

10.3791/3122-v

DOI:
10.3791/3122-v

•

10:09 min
January 03, 2012

•

5 Views
,
1Centre for Immunity, Infection and Evolution,University of Edinburgh

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An in vitro model for cerebral malaria sequestration is described1. Plasmodium falciparum infected red blood cells are selected for binding to immortalized human brain microvascular endothelial cells. The selected parasites show a distinct phenotype. The selection process can be applied using various P. falciparum strains and endothelial cell lines.

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Plasmodium FalciparumCytoadhesionHuman Brain Endothelial CellsCerebral MalariaMicrovasculatureSequestrationBlood Vessel WallsHBEC-5iIn Vitro ModelPanning AssayHigh-binding ParasitesPigmented Trophozoite Stage

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Claessens, A., Rowe, J. A. Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells. J. Vis. Exp. (59), e3122, doi:10.3791/3122 (2012).

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