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Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells

Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells

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10:09 min

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January 03, 2012

DOI:

10.3791/3122-v

DOI:
10.3791/3122-v

•

10:09 min
January 03, 2012

•

5 Views
,
1Centre for Immunity, Infection and Evolution,University of Edinburgh

Summary

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An in vitro model for cerebral malaria sequestration is described1. Plasmodium falciparum infected red blood cells are selected for binding to immortalized human brain microvascular endothelial cells. The selected parasites show a distinct phenotype. The selection process can be applied using various P. falciparum strains and endothelial cell lines.

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Plasmodium FalciparumCytoadhesionHuman Brain Endothelial CellsCerebral MalariaMicrovasculatureSequestrationBlood Vessel WallsHBEC-5iIn Vitro ModelPanning AssayHigh-binding ParasitesPigmented Trophozoite Stage

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Cite this Article

Claessens, A., Rowe, J. A. Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells. J. Vis. Exp. (59), e3122, doi:10.3791/3122 (2012).

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