Isolation of Human Islets from Partially Pancreatectomized Patients

Gregor B&#246;tticher; Doroth&#232;e Sturm; Florian Ehehalt; Klaus P. Knoch; Stephan Kersting; Robert Gr&#252;tzmann; Gustavo B. Baretton; Michele Solimena; Hans D. Saeger

doi:10.3791/2962

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Isolation of Human Islets from Partially Pancreatectomized Patients

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Isolation of Human Islets from Partially Pancreatectomized Patients

15,625 Views

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11:10 min

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July 30, 2011

DOI:

10.3791/2962-v

DOI:

10.3791/2962-v

•

11:10 min

July 30, 2011

•

9 Views

Gregor Bötticher¹, Dorothèe Sturm¹, Florian Ehehalt¹, Klaus P. Knoch², Stephan Kersting¹, Robert Grützmann¹, Gustavo B. Baretton³, Michele Solimena², Hans D. Saeger¹

¹Department of GI-, Thorax- and Vascular Surgery,University Hospital Carl Gustav Carus, University of Technology Dresden, ²Molecular Diabetology,Paul Langerhans Institute Dresden, ³Patoloji Bölümü,University Hospital Carl Gustav Carus, University of Technology Dresden

Özet

The supply of type 2 diabetic islets for research is insufficient. Here we share our protocol for isolating islets from patients undergoing partial pancreatectomy. This approach represents a unique venue for obtaining islets from type 2 diabetic and clinically matched non-diabetic subjects in adequate numbers for basic and clinical studies.

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Isolation of Human Islets from Partially Pancreatectomized Patients

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