'Dergi'
/
Ormancılık ve botanik
/
In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
JoVE Journal
Ormancılık ve botanik
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.'  'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
JoVE Journal Ormancılık ve botanik
In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

DOI:
10.3791/2783-v

•

05:57 min

•

May 16, 2011

•

, , , ,
1Universite de Montreal,Groupe de Recherche sur les Maladies Infectieuses du Porc GREMIP, Faculte de medecine veterinaire

'Bölümler'

  • 00:05'Başlık'
  • 00:58Prepare Bacterial Strains and Epithelial Cells
  • 02:34Infection of Cells
  • 04:15Representative cfu Counting Results
  • 05:35Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

This protocol is a simple bacterial adhesion assay consisting in counting the numbers of bacterial colony forming units that are adhered onto cultured cells. The assay is robust, independent of the adhesin studied, and numerous variations are used in most laboratories working on bacterial pathogenesis.

'Etiketler'

In Vitro AssayBacterial AdhesionMammalian Epithelial CellsBacterial PathogensAdhesinsHost Cell Surface ReceptorsCellular MicrobiologyBacterial Pathogenesis StudiesAdherence AssayEscherichia Coli Strain 27875Autotransporter Adhesin Involved In Diffuse Adherence (AIDA)AidA GeneHEp-2 Human Epithelial Cell Line

Article

Embed

'ÇALMA LİSTESİNE EKLE'

Usage Statistics

'İlgili Videolar'

Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells

Introducing Shear Stress in the Study of Bacterial Adhesion

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model

In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

Human Neutrophil Flow Chamber Adhesion Assay

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Electroporation of Functional Bacterial Effectors into Mammalian Cells

In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

Read Article