/
/
In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
25,051 Views
•
05:57 min
•
May 16, 2011
DOI:
Summary
Automatically generated
This protocol is a simple bacterial adhesion assay consisting in counting the numbers of bacterial colony forming units that are adhered onto cultured cells. The assay is robust, independent of the adhesin studied, and numerous variations are used in most laboratories working on bacterial pathogenesis.
Explore More Videos
In Vitro AssayBacterial AdhesionMammalian Epithelial CellsBacterial PathogensAdhesinsHost Cell Surface ReceptorsCellular MicrobiologyBacterial Pathogenesis StudiesAdherence AssayEscherichia Coli Strain 27875Autotransporter Adhesin Involved In Diffuse Adherence (AIDA)AidA GeneHEp-2 Human Epithelial Cell Line
Read Article
Cite this Article
Letourneau, J., Levesque, C., Berthiaume, F., Jacques, M., Mourez, M. In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells. J. Vis. Exp. (51), e2783, doi:10.3791/2783 (2011).
Copy