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In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
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In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
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05:57 min
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May 16, 2011
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Summary
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This protocol is a simple bacterial adhesion assay consisting in counting the numbers of bacterial colony forming units that are adhered onto cultured cells. The assay is robust, independent of the adhesin studied, and numerous variations are used in most laboratories working on bacterial pathogenesis.
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Letourneau, J., Levesque, C., Berthiaume, F., Jacques, M., Mourez, M. In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells. J. Vis. Exp. (51), e2783, doi:10.3791/2783 (2011).
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