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Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues

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Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues

JoVE Journal
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JoVE Journal Biyoloji

Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues

Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues

Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues

DOI:

10.3791/200322-v

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03:38 min

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July 28, 2023

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Lucas Stalder, Fabian Koechl, Kerstin Hahn, Marc Sultan, Megana K. Prasad

¹Roche Pharma Research and Early Development, Pharmaceutical Sciences,Roche Innovation Centre Basel

Etiketler

Single Nuclei Sucrose Gradient Cleanup Nuclei Suspension SCS Centrifugation Propidium Iodide Staining Cell Counter Single Nuclei RNA Sequencing Gene Counts UMI Counts Mitochondrial Content Cellular Proportions Technical Variability

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