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Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
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Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues (Video) | JoVE
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Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues

Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues (Video) | JoVE

Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues

DOI:
10.3791/200322-v

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03:38 min

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July 28, 2023

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, , , ,
1Roche Pharma Research and Early Development, Pharmaceutical Sciences,Roche Innovation Centre Basel

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Keywords: Single NucleiSucrose Gradient CleanupNuclei SuspensionSCSCentrifugationPropidium Iodide StainingCell CounterSingle Nuclei RNA SequencingGene CountsUMI CountsMitochondrial ContentCellular ProportionsTechnical Variability

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