Journal
/
生物学
/
Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
/
Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues

Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues

242 Views

•

03:38 min

•

July 28, 2023

DOI:

10.3791/200322-v

DOI:
10.3791/200322-v

•

03:38 min
July 28, 2023

•

1 Views
, , , ,
1Roche Pharma Research and Early Development, Pharmaceutical Sciences,Roche Innovation Centre Basel

Explore More Videos

Single NucleiSucrose Gradient CleanupNuclei SuspensionSCSCentrifugationPropidium Iodide StainingCell CounterSingle Nuclei RNA SequencingGene CountsUMI CountsMitochondrial ContentCellular ProportionsTechnical Variability

Read Article

Cite this Article

Stalder, L., Koechl, F., Hahn, K., Sultan, M., Prasad, M. K. Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues. J. Vis. Exp. (Pending Publication), e200322, doi:10.3791/200322 (2023).

Download .ris file

Copy

Share Video

.

Copy