Dergi
/
Biyoloji
/
Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
JoVE Journal
Biyoloji
This content is Free Access.
JoVE Journal Biyoloji
Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

DOI:
10.3791/1630-v

•

09:23 min

•

December 21, 2009

•

, ,
1Joint Graduate Program in Cell and Developmental Biology,UMDNJ-Graduate School of Biomedical Sciences and Rutgers: The State University of New Jersey, 2Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School,University of Medicine and Dentistry of New Jersey

Bölümler

  • 00:00Başlık
  • 01:20Introduction
  • 01:54Preparation of Drosophila Embryos for Transmission Electron Microscopy
  • 08:32Representative Electron Microscopy Data
  • 08:58Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We describe a process for fixation, embedding, sectioning, and imaging of late stage Drosophila embryos for Trasmission Electron Microscopy of the embryonic heart tube. This technique allows for the visualization of the heart tube lumen as well as the basement membrane, which lines the lumen of the heart.

Etiketler

Electron MicroscopyDrosophila Embryonic Heart TubeCell MigrationCell-cell AdhesionCell Shape ChangesEmbryonic DevelopmentLumen VisualizationMembrane FeaturesWhole Mount EmbryosTransmission Electron MicroscopyFixationEmbeddingSectioningLate Stage Drosophila Embryos

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Preparation of Neuronal Cultures from Midgastrula Stage Drosophila Embryos

The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol

Fluorescent Labeling of Drosophila Heart Structures

Visualizing the Beating Heart in Drosophila

Preparation of Drosophila S2 cells for Light Microscopy

A Novel Ex vivo Culture Method for the Embryonic Mouse Heart

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)

Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy

Miniaturized Sample Preparation for Transmission Electron Microscopy

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

Read Article