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Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
JoVE 杂志
生物学
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JoVE 杂志 生物学
Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

DOI:
10.3791/1630-v

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09:23 min

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December 21, 2009

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, ,
1Joint Graduate Program in Cell and Developmental Biology,UMDNJ-Graduate School of Biomedical Sciences and Rutgers: The State University of New Jersey, 2Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School,University of Medicine and Dentistry of New Jersey

Chapters

  • 00:00Title
  • 01:20Introduction
  • 01:54Preparation of Drosophila Embryos for Transmission Electron Microscopy
  • 08:32Representative Electron Microscopy Data
  • 08:58Conclusion

Summary

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We describe a process for fixation, embedding, sectioning, and imaging of late stage Drosophila embryos for Trasmission Electron Microscopy of the embryonic heart tube. This technique allows for the visualization of the heart tube lumen as well as the basement membrane, which lines the lumen of the heart.

Tags

Electron MicroscopyDrosophila Embryonic Heart TubeCell MigrationCell-cell AdhesionCell Shape ChangesEmbryonic DevelopmentLumen VisualizationMembrane FeaturesWhole Mount EmbryosTransmission Electron MicroscopyFixationEmbeddingSectioningLate Stage Drosophila Embryos

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