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Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
JoVE Journal
Biologie
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JoVE Journal Biologie
Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

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09:23 min

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December 21, 2009

DOI:

10.3791/1630-v

DOI:
10.3791/1630-v

•

09:23 min
December 21, 2009

•

2 Views
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1Joint Graduate Program in Cell and Developmental Biology,UMDNJ-Graduate School of Biomedical Sciences and Rutgers: The State University of New Jersey, 2Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School,University of Medicine and Dentistry of New Jersey

Summary

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We describe a process for fixation, embedding, sectioning, and imaging of late stage Drosophila embryos for Trasmission Electron Microscopy of the embryonic heart tube. This technique allows for the visualization of the heart tube lumen as well as the basement membrane, which lines the lumen of the heart.

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Electron MicroscopyDrosophila Embryonic Heart TubeCell MigrationCell-cell AdhesionCell Shape ChangesEmbryonic DevelopmentLumen VisualizationMembrane FeaturesWhole Mount EmbryosTransmission Electron MicroscopyFixationEmbeddingSectioningLate Stage Drosophila Embryos

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Cite this Article

Soplop, N. H., Patel, R., Kramer, S. G. Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube. J. Vis. Exp. (34), e1630, doi:10.3791/1630 (2009).

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