We have modified the conditions for DFAT cell generation and provide herein information regarding the use of an improved growth medium for the production of these cells.
This research was supported in part by Program for Creating Start-ups from Advanced Research and Technology (START Program) from the Japan Society for the Promotion of Science (ST261006IP, TM) and by Program for the Strategic Research Foundation at Private Universities (2014-2019) (S1411018, TM) from the Ministry of Education, Sports, Science and Technology.
Materials
CSTI303-MSC medium
CSTI
87-671
This medium is defined as DCM in the text
PBS(-)
Wako
166-23555
It does not contain Mg2+ and Ca2+
DMEM medium
Gibco
11965-092
Fetal Bovine Serum
Sigma
172012
Collagenase type II
Sigma
C-6885
Scissors
Takasago Medical Industry Co., Ltd
TKZ-F2194-1
Shaker
TAITEC
Bioshaker V.BR-36
Falcon Cell Strainer 100um Yellow
CORNING LIFE SCIENCES
DL 352360
Falcon 12.5cm² Rectangular Canted Neck Cell Culture Flask with Blue Vented Screw Cap
Taniguchi, H., Kazama, T., Hagikura, K., Yamamoto, C., Kazama, M., Nagaoka, Y., Matsumoto, T. An Efficient Method to Obtain Dedifferentiated Fat Cells. J. Vis. Exp. (113), e54177, doi:10.3791/54177 (2016).