This study describes the development of a modified CsCl method that easily purifies T. gondii oocysts from feces of infected cats that are suitable for molecular biological and tissue culture manipulation
We would like to thank Nichole Brinkman and Michael Ware for technical review of the manuscript and Dr. Abu Sayed and Linda Ransick for technical and administrative support. The United States Environmental Protection Agency through its Office of Research and Development partially funded and collaborated in the research described here under contract number EP-D-06-096 to Dynamac, Inc. and an interagency agreement number DW-12-92289801-0 to USDA. It has been subjected to Agency review and approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. MJS current address: Dynamac, Inc. Cincinnati, OH.