JOVE JOURNAL

Immunology and Infection

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Journal (Immunology and Infection)

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings

Yan D. Niu¹, Jae Eun Hyun¹, Nghi Nguyen¹

¹Department of Ecosystem and Public Health, Faculty of Veterinary Medicine,University of Calgary

Journal (Immunology and Infection)

Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy

Liam Wilkinson^1,2, Johnathan Canton^2,3

¹Department of Biochemistry & Microbiology, Faculty of Science,University of Victoria, ²Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Science,University of Calgary, ³Calvin, Joan and Phoebe Snyder Institute for Chronic Diseases,University of Calgary

Journal (Immunology and Infection)

An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis

Seónadh O’Leary¹, Ahmad Z. Bahlool^1,2, Gemma O’Connor², Sally-Ann Cryan^2,3,4, Joseph M. Keane¹, Mary P. O’Sullivan¹

¹Department of Clinical Medicine, Trinity Translational Medicine Institute, St. James's Hospital, Trinity College Dublin,The University of Dublin, ²School of Pharmacy and Biomolecular Sciences,Royal College of Surgeons in Ireland (RCSI), ³SFI Advanced Materials and Bioengineering Research (AMBER) Centre,RCSI & TCD, ⁴SFI Centre for Research in Medical Devices (CURAM), RCSI,Dublin and National University of Ireland

Journal (Immunology and Infection)

Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria

Emily Morgan¹, Jonah Faye Longares¹, Marie-Anne Félix², Robert J. Luallen¹

¹Department of Biology,San Diego State University, ²Institut de Biologie de l’École Normale Supérieure

Journal (Immunology and Infection)

Retrograde Parotid Gland Infusion through Stensen’s Duct in a Non-Human Primate for Vectored Gene Delivery

Guy El Helou¹, Joseph F. Goodman², Maria Blevins³, David L. Caudell⁴, Todd A. Ponzio³, John W. Sanders^3,5

¹Department of Medicine, Division of Infectious Diseases and Global Medicine,University of Florida, ²Department of Otolaryngology,George Washington University School of Medicine, ³Department of Medicine, Section on Infectious Diseases,Wake Forest Baptist Medical Center, ⁴Department of Pathology,Wake Forest School of Medicine, ⁵Department of Medicine, Section of Infectious Diseases,Hefner Veterans Affairs Medical Center

Journal (Immunology and Infection)

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Reza Rezaei^1,2, Abera Surendran^1,2, Ragunath Singaravelu^1,2, Taylor R. Jamieson^1,2, Parisa Taklifi³, Joanna Poutou^1,2, Taha Azad^1,2, Carolina S. Ilkow^1,2

¹Ottawa Hospital Research Institute, ²Department of Biochemistry, Microbiology and Immunology,University of Ottawa, ³Department of Biotechnology,University of Tehran

Journal (Immunology and Infection)

Assessing Biofilm Dispersal in Murine Wounds

Whitni K. Redman*^1,2, Garrett S. Welch*^1,3, Kendra P. Rumbaugh^1,2,3

¹Department of Surgery,Texas Tech University Health Sciences Center, ²Immunology and Molecular Microbiology,Texas Tech University Health Sciences Center, ³TTUHSC Surgery Burn Center of Research Excellence,Texas Tech University Health Sciences Center

Journal (Immunology and Infection)

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Katharina M. Scheurlen¹, Dylan L. Snook¹, Sarah A. Gardner¹, Maurice R. Eichenberger¹, Susan Galandiuk¹

¹Price Institute of Surgical Research, Department of Surgery,University of Louisville

Journal (Immunology and Infection)

Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (Oryctolagus cuniculus)

Chelsea D. Landon^1,2, Gabriella Dancourt¹, Vivian Shing², Herman F. Staats^2,3,4

¹Division of Laboratory Animal Resources,Duke University Medical Center, ²Department of Pathology,Duke University Medical Center, ³Department of Immunology,Duke University Medical Center, ⁴Duke Human Vaccine Institute,Duke University Medical Center

Journal (Immunology and Infection)

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Anil Kumar¹, Surekha P.¹, Sahil Gulati², Somnath Dutta¹

¹Molecular Biophysics Unit,Indian Institute of Science, ²Gatan Inc.

Journal (Immunology and Infection)

Investigating the Phagocytosis of Leishmania using Confocal Microscopy

Amanda R. Paixão*¹, Beatriz R. S. Dias*¹, Luana C. Palma¹, Natália M. Tavares¹, Cláudia I. Brodskyn¹, Juliana P. B de Menezes¹, Patricia S. T. Veras^1,2

¹Laboratory of Host-Parasite Interaction and Epidemiology,Gonçalo Moniz Institute, ²National Institute of Science and Technology of Tropical Diseases - National Council for Scientific Research and Development (CNPq)

Journal (Immunology and Infection)

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Jodi F. Evans¹, Anthony E. Ricigliano¹, Anthony V. Morante¹, Emily Martinez¹, Darlisy Vargas¹, Jonah Thyagaraj¹

¹Molloy College

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