JOVE JOURNAL

Immunology and Infection

Showing 349 - 360 in 1,529 videos.

Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis

Md Huzzatul Mursalin*^1,2, Erin Livingston*¹, Phillip S. Coburn^2,3, Frederick C. Miller⁴, Roger Astley^2,3, Michelle C. Callegan^1,2,3

¹Department of Microbiology and Immunology,University of Oklahoma Health Sciences Center, ²Department of Ophthalmology,Dean McGee Eye Institute, ³Dean McGee Eye Institute, ⁴Department of Cell Biology and Department of Family and Preventive Medicine,University of Oklahoma Health Sciences Center

Journal (Immunology and Infection)

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

Ruichen Sun¹, Daniel Brogan¹, Anna Buchman¹, Ting Yang¹, Omar S. Akbari¹

¹Division of Biological Sciences, Section of Cell and Developmental Biology,University of California

Journal (Immunology and Infection)

Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes

David C. Montrose^1,2, Erin M. McNally³, Erika Sue³, Andrew J. Dannenberg³

¹Department of Pathology, Renaissance School of Medicine,Stony Brook University, Stony Brook, NY, ²Stony Brook Cancer Center, Stony Brook, NY, ³Department of Medicine,Weill Cornell Medicine, New York, NY

Journal (Immunology and Infection)

Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat

Lavinia Curini*^1,2, Mary Roxana Christopher*¹, Herko Grubitzsch^3,4, Ulf Landmesser^1,3, Amedeo Amedei*^2,5, Alexander Lauten*^1,6, Brunilda Alushi*^1,6

¹Department of Cardiology, Campus Benjamin Franklin,Charité Universitätsmedizin Berlin and German Centre for Cardiovascular Research (DZHK), ²Department of Experimental and Clinical Medicine,University of Florence, ³Berlin Institute of Health, ⁴Department of Cardiology,German Heart Centre Berlin (DHZB), ⁵Sod of Interdisciplinary Internal Medicine,Azienda Ospedaliera Universitaria Careggi (AOUC), ⁶Department of General and Interventional Cardiology,Helios Klinikum Erfurt

Journal (Immunology and Infection)

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

Xia Meng*^1,2, Weiwei Cui*¹, Xianchen Meng¹, Jianye Wang^1,2, Jinqiu Wang³, Guoqiang Zhu^1,2

¹College of Veterinary Medicine,Yangzhou University, ²Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, ³Department of Animal Husbandry and Veterinary Medicine,Beijing Agricultural Vocational College

Journal (Immunology and Infection)

Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung

Niamh E. Harrington*¹, Esther Sweeney*¹, Ilyas Alav^1,2, Freya Allen¹, John Moat^1,3, Freya Harrison¹

¹School of Life Science,University of Warwick, ²Institute of Microbiology and Infection,University of Birmingham, ³Warwick Antimicrobial Screening Facility, School of Life Science,University of Warwick

Journal (Immunology and Infection)

Flow Cytometric Characterization of Murine B Cell Development

Faith M. Harris*¹, Karoline A. Meagher*¹, Maggie Zhong¹, Benjamin J. Daniel², Mark Eckersdorff¹, Jesse A. Green¹, Vera Voronina¹, Chunguang Guo¹, Andre Limnander³, Lynn E. Macdonald¹

¹Regeneron Tech Centers,Regeneron Pharmaceuticals, Inc., ²Regeneron Therapeutic Proteins,Regeneron Pharmaceuticals, Inc., ³Immunology and Inflammation,Regeneron Pharmaceuticals, Inc.

Journal (Immunology and Infection)

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Lael Werner^1,2, Chen Dor^3,4, Naomi Salamon¹, Meital Nagar^3,4, Dror S. Shouval^1,2

¹Pediatric Gastroenterology Unit, Edmond and Lily Safra Children's Hospital,Sheba Medical Center, ²Sackler Faculty of Medicine,Tel Aviv University, ³Cancer Research Center,Sheba Medical Center, ⁴Molecular Hematology Laboratory,Sheba Medical Center

Journal (Immunology and Infection)

A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice

Sonia Simonetti*^1,2, Ambra Natalini*^1,2, Giovanna Peruzzi³, Alfredo Nicosia⁴, Antonella Folgori⁵, Stefania Capone⁵, Angela Santoni^2,6, Francesca Di Rosa¹

¹Institute of Molecular Biology and Pathology,National Research Council of Italy (CNR), ²Department of Molecular Medicine,University of Rome “Sapienza”, ³Center for Life Nano Science,Istituto Italiano di Tecnologia, ⁴Department of Molecular Medicine and Medical Biotechnology,University of Naples Federico II, ⁵Reithera Srl, ⁶IRCCS, Neuromed

Journal (Immunology and Infection)

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Anna Kula-Pacurar¹, Jakub Wadas², Agnieszka Suder¹, Artur Szczepanski^1,2, Aleksandra Milewska^1,2, Marek Ochman^3,4, Tomasz Stacel^3,4, Krzysztof Pyrc¹

¹Malopolska Centre of Biotechnology,Jagiellonian University, ²Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology,Jagiellonian University, ³Department of Cardiac, Vascular and Endovascular Surgery and Transplantology,The Medical University of Silesia in Katowice, ⁴Silesian Centre for Heart Diseases

Journal (Immunology and Infection)

Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software

Amanda J. Morris¹, Alvin Li¹, Lindsay Jackson¹, Yvonne C. W. Yau^1,2, Valerie Waters^1,3

¹Translational Medicine,Hospital for Sick Children, ²Microbiology, Department of Pediatric Laboratory Medicine,Hospital for Sick Children, ³Infectious Diseases, Department of Pediatrics,Hospital for Sick Children

Journal (Immunology and Infection)

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

Valerie P. O'Brien^1,2,6, Matthew S. Joens^3,7, Amanda L. Lewis^1,2,4,8, Nicole M. Gilbert^2,5

¹Department of Molecular Microbiology,Washington University School of Medicine in Saint Louis, ²Center for Women’s Infectious Disease Research,Washington University School of Medicine in Saint Louis, ³Center for Cellular Imaging,Washington University School of Medicine in Saint Louis, ⁴Department of Obstetrics and Gynecology,Washington University School of Medicine in Saint Louis, ⁵Department of Pediatrics,Washington University School of Medicine in Saint Louis, ⁶Fred Hutchinson Cancer Research Center, ⁷TESCAN USA, Inc., ⁸University of California San Diego

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