JOVE JOURNAL

Immunology and Infection

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Rescue of Recombinant Newcastle Disease Virus from cDNA

Juan Ayllon^1,2, Adolfo García-Sastre^1,2,3, Luis Martínez-Sobrido⁴

¹Department of Microbiology,Icahn School of Medicine at Mount Sinai, ²Global Health and Emerging Pathogens Institute,Icahn School of Medicine at Mount Sinai, ³Department of Medicine,Icahn School of Medicine at Mount Sinai, ⁴Department of Microbiology and Immunology, School of Medicine and Dentistry,University of Rochester

Journal (Immunology and Infection)

Assays for the Identification of Novel Antivirals against Bluetongue Virus

Linlin Gu¹, Stewart W. Schneller², Qianjun Li¹

¹Division of Infectious Diseases, Department of Medicine,University of Alabama at Birmingham, ²Molette Laboratory for Drug Discovery, Department of Chemistry and Biochemistry,Auburn University

Journal (Immunology and Infection)

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Simone K. Bedoya*¹, Tenisha D. Wilson*¹, Erin L. Collins¹, Kenneth Lau¹, Joseph Larkin III¹

¹Department of Microbiology and Cell Science,The University of Florida

Journal (Immunology and Infection)

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

Andreas Kühbacher^1,2,3, Edith Gouin^1,2,3, Jason Mercer⁴, Mario Emmenlauer⁵, Christoph Dehio⁵, Pascale Cossart^1,2,3, Javier Pizarro-Cerdá^1,2,3

¹Unité des Interactions Bactéries Cellules,Pasteur Institute, ²INSERM U604, ³Institut National de la Recherche Agronomique (INRA), USC2020, ⁴Institute of Biochemistry,ETH Zürich, ⁵Focal Area Infection Biology, Biozentrum,University of Basel

Journal (Immunology and Infection)

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

Christina Stoeckle^1,2, Ioanna A. Rota¹, Eva Tolosa³, Christoph Haller⁴, Arthur Melms⁵, Eleni Adamopoulou¹

¹Department of General Neurology,Hertie Institute for Clinical Brain Research, ²Institute of Pharmacology,University of Bern, ³Department of Immunology,University Medical Center Hamburg-Eppendorf, ⁴Department of Thoracic and Cardiovascular Surgery,University Clinic Tuebingen, ⁵Department of Neurology,University Hospital Erlangen

Journal (Immunology and Infection)

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment

Adrianne N. Edwards¹, Jose M. Suárez¹, Shonna M. McBride¹

¹Department of Microbiology and Immunology,Emory University School of Medicine

Journal (Immunology and Infection)

Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish

Michelle F. Goody¹, Eric Peterman¹, Con Sullivan¹, Carol H. Kim¹

¹Department of Molecular and Biomedical Sciences,University of Maine

Journal (Immunology and Infection)

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Jo-Ann McClure-Warnier¹, John M. Conly^1,2,3,4,5, Kunyan Zhang^1,2,3,4,5

¹Centre for Antimicrobial Resistance,Alberta Health Services / Calgary Laboratory Services / University of Calgary, ²Department of Pathology & Laboratory Medicine,University of Calgary, ³Department of Microbiology, Immunology and Infectious Diseases,University of Calgary, ⁴Department of Medicine,University of Calgary, ⁵The Calvin, Phoebe and Joan Snyder Institute for Chronic Diseases,University of Calgary

Journal (Immunology and Infection)

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

M. Brittany Johnson¹, Alison K. Criss¹

¹Department of Microbiology, Immunology, and Cancer Biology,University of Virginia Health Sciences Center

Journal (Immunology and Infection)

Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research

Monica E. Embers¹, Britton J. Grasperge¹, Mary B. Jacobs¹, Mario T. Philipp¹

¹Division of Bacteriology & Parasitology, Tulane National Primate Research Center,Tulane University Health Sciences Center

Journal (Immunology and Infection)

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

Muthappa Senthil-Kumar¹, Hee-Kyung Lee¹, Kirankumar S. Mysore¹

¹Plant Biology Division,The Samuel Roberts Noble Foundation

Journal (Immunology and Infection)

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

Kevin Wilhelmsen¹, Katherine Farrar^1,2, Judith Hellman¹

¹Department of Anesthesia and Perioperative Care,University of California, San Francisco, ²Graduate Program in Biomedical Sciences,University of California, San Francisco

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