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JOVE JOURNAL
Genetics
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Journal (Genetics)
Hybrid
De Novo
Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Belle M. Sharon
1
,
Neha V. Hulyalkar
1
,
Vivian H. Nguyen
1
,
Philippe E. Zimmern
2
,
Kelli L. Palmer
1
,
Nicole J. De Nisco
1
1
Department of Biological Sciences
,
University of Texas at Dallas
,
2
Department of Urology
,
University of Texas Southwestern Medical Center
Author
Belle M. Sharon
1
,
Neha V. Hulyalkar
1
,
Vivian H. Nguyen
1
,
Philippe E. Zimmern
2
,
Kelli L. Palmer
1
,
Nicole J. De Nisco
1
Institution
1
Department of Biological Sciences
,
University of Texas at Dallas
,
2
Department of Urology
,
University of Texas Southwestern Medical Center
AUTHORS
Journal (Genetics)
Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of
Leptospira
L. G. V. Fernandes
1,2
,
R. L. Hornsby
1
,
A.L.T.O. Nascimento*
2
,
J. E. Nally*
1
1
Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service
,
United States Department of Agriculture
,
2
Laboratório de Desenvolvimento de Vacinas
,
Instituto Butantan
Author
L. G. V. Fernandes
1,2
,
R. L. Hornsby
1
,
A.L.T.O. Nascimento*
2
,
J. E. Nally*
1
Institution
1
Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service
,
United States Department of Agriculture
,
2
Laboratório de Desenvolvimento de Vacinas
,
Instituto Butantan
AUTHORS
Journal (Genetics)
Application of DNA Fingerprinting using the
D1S80
Locus in Lab Classes
Meike Siebers*
1
,
Agatha Walla*
1,2
,
Thea Rütjes
1
,
Moritz-Fabian Müller
4
,
Maria von Korff
1,2,3
1
Institute of Plant Genetics
,
Heinrich-Heine-University
,
2
Cluster of Excellence on Plant Sciences "SMART Plants for Tomorrow's Needs"
,
3
Max Planck Institute for Plant Breeding Research
,
4
IBG-1: Biotechnology, Forschungszentrum Jülich GmbH
Author
Meike Siebers*
1
,
Agatha Walla*
1,2
,
Thea Rütjes
1
,
Moritz-Fabian Müller
4
,
Maria von Korff
1,2,3
Institution
1
Institute of Plant Genetics
,
Heinrich-Heine-University
,
2
Cluster of Excellence on Plant Sciences "SMART Plants for Tomorrow's Needs"
,
3
Max Planck Institute for Plant Breeding Research
,
4
IBG-1: Biotechnology, Forschungszentrum Jülich GmbH
AUTHORS
Journal (Genetics)
Microinjection Method for
Anopheles gambiae
Embryos
Rebeca Carballar-Lejarazú
1
,
Taylor Tushar
1
,
Thai Binh Pham
2
,
Anthony A. James
1,2
1
Department of Microbiology & Molecular Genetics
,
University of California, Irvine
,
2
Department of Molecular Biology & Biochemistry
,
University of California, Irvine
Author
Rebeca Carballar-Lejarazú
1
,
Taylor Tushar
1
,
Thai Binh Pham
2
,
Anthony A. James
1,2
Institution
1
Department of Microbiology & Molecular Genetics
,
University of California, Irvine
,
2
Department of Molecular Biology & Biochemistry
,
University of California, Irvine
AUTHORS
Journal (Genetics)
Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations
Rebeca Carballar-Lejarazú
1
,
Thai Binh Pham
2
,
Adam Kelsey
1
,
Taylor Tushar
1
,
Anthony A. James
1,2
1
Department of Microbiology & Molecular Genetics
,
University of California, Irvine
,
2
Department of Molecular Biology & Biochemistry
,
University of California, Irvine
Author
Rebeca Carballar-Lejarazú
1
,
Thai Binh Pham
2
,
Adam Kelsey
1
,
Taylor Tushar
1
,
Anthony A. James
1,2
Institution
1
Department of Microbiology & Molecular Genetics
,
University of California, Irvine
,
2
Department of Molecular Biology & Biochemistry
,
University of California, Irvine
AUTHORS
Journal (Genetics)
Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in
Magnaporthe oryzae
Zechi Wu*
1
,
Wanyu Sun*
1
,
Sida Zhou
1
,
Li Zhang
1
,
Xinyu Zhao
1
,
Yang Xu
1
,
Weixiang Wang
1
1
Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Department of Agronomy
,
Beijing University of Agriculture
Author
Zechi Wu*
1
,
Wanyu Sun*
1
,
Sida Zhou
1
,
Li Zhang
1
,
Xinyu Zhao
1
,
Yang Xu
1
,
Weixiang Wang
1
Institution
1
Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Department of Agronomy
,
Beijing University of Agriculture
AUTHORS
Journal (Genetics)
Culture and Assay of Large-Scale Mixed-Stage
Caenorhabditis elegans
Populations
Amanda O. Shaver
1
,
Goncalo J. Gouveia
2
,
Pamela S. Kirby
3
,
Erik C. Andersen
4
,
Arthur S. Edison
1,2,3
1
Department of Genetics
,
University of Georgia
,
2
Department of Biochemistry and Molecular Biology
,
University of Georgia
,
3
Complex Carbohydrate Research Center
,
University of Georgia
,
4
Department of Molecular Biosciences
,
Northwestern University
Author
Amanda O. Shaver
1
,
Goncalo J. Gouveia
2
,
Pamela S. Kirby
3
,
Erik C. Andersen
4
,
Arthur S. Edison
1,2,3
Institution
1
Department of Genetics
,
University of Georgia
,
2
Department of Biochemistry and Molecular Biology
,
University of Georgia
,
3
Complex Carbohydrate Research Center
,
University of Georgia
,
4
Department of Molecular Biosciences
,
Northwestern University
AUTHORS
Journal (Genetics)
Small-Cage Laboratory Trials of Genetically-Engineered Anopheline Mosquitoes
Rebeca Carballar-Lejarazú
1
,
Thai Binh Pham
2
,
Vanessa Bottino-Rojas
1
,
Adriana Adolfi
1,3
,
Anthony A. James
1,2
1
Department of Microbiology & Molecular Genetics
,
University of California, Irvine
,
2
Department of Molecular Biology & Biochemistry
,
University of California, Irvine
,
3
Vector Biology Department
,
Liverpool School of Tropical Medicine
Author
Rebeca Carballar-Lejarazú
1
,
Thai Binh Pham
2
,
Vanessa Bottino-Rojas
1
,
Adriana Adolfi
1,3
,
Anthony A. James
1,2
Institution
1
Department of Microbiology & Molecular Genetics
,
University of California, Irvine
,
2
Department of Molecular Biology & Biochemistry
,
University of California, Irvine
,
3
Vector Biology Department
,
Liverpool School of Tropical Medicine
AUTHORS
Journal (Genetics)
Using the GAL4-UAS System for Functional Genetics in
Anopheles gambiae
Beth Crawford Poulton
1
,
Fraser Colman
1
,
Amalia Anthousi
1,2,3
,
Linda Grigoraki
1
,
Adriana Adolfi
1
,
Amy Lynd
1
,
Gareth John Lycett
1
1
Department of Vector Biology
,
Liverpool School of Tropical Medicine
,
2
IMBB FORTH
,
3
Department of Biology
,
University of Crete
Author
Beth Crawford Poulton
1
,
Fraser Colman
1
,
Amalia Anthousi
1,2,3
,
Linda Grigoraki
1
,
Adriana Adolfi
1
,
Amy Lynd
1
,
Gareth John Lycett
1
Institution
1
Department of Vector Biology
,
Liverpool School of Tropical Medicine
,
2
IMBB FORTH
,
3
Department of Biology
,
University of Crete
AUTHORS
Journal (Genetics)
Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders
Camila F. Almeida
1
,
Emma C. Frair
1
,
Nianyuan Huang
1
,
Reid Neinast
2
,
Kim L. McBride
2,3,4,6
,
Robert B. Weiss
5
,
Kevin M. Flanigan
1,6
,
Nicolas Wein
1,6
1
Center for Gene Therapy
,
The Research Institute at Nationwide Children’s Hospital
,
2
Center for Cardiovascular Research
,
The Research Institute at Nationwide Children’s Hospital
,
3
The Heart Center
,
Nationwide Children’s Hospital
,
4
Division of Genetic and Genomic Medicine
,
Nationwide Children’s Hospital
,
5
Department of Human Genetics
,
The University of Utah School of Medicine
,
6
Department of Pediatrics
,
The Ohio State University
Author
Camila F. Almeida
1
,
Emma C. Frair
1
,
Nianyuan Huang
1
,
Reid Neinast
2
,
Kim L. McBride
2,3,4,6
,
Robert B. Weiss
5
,
Kevin M. Flanigan
1,6
,
Nicolas Wein
1,6
Institution
1
Center for Gene Therapy
,
The Research Institute at Nationwide Children’s Hospital
,
2
Center for Cardiovascular Research
,
The Research Institute at Nationwide Children’s Hospital
,
3
The Heart Center
,
Nationwide Children’s Hospital
,
4
Division of Genetic and Genomic Medicine
,
Nationwide Children’s Hospital
,
5
Department of Human Genetics
,
The University of Utah School of Medicine
,
6
Department of Pediatrics
,
The Ohio State University
AUTHORS
Journal (Genetics)
Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant
Harpegnathos saltator
Kayli Sieber
1
,
Maya Saar
1
,
Comzit Opachaloemphan
2
,
Matthew Gallitto
3
,
Huan Yang
2
,
Hua Yan
1,4
1
Department of Biology
,
University of Florida
,
2
Department of Biochemistry and Molecular Pharmacology
,
New York University School of Medicine
,
3
Department of Radiation Oncology
,
Columbia University Medical Center
,
4
Center for Smell and Taste
,
University of Florida
Author
Kayli Sieber
1
,
Maya Saar
1
,
Comzit Opachaloemphan
2
,
Matthew Gallitto
3
,
Huan Yang
2
,
Hua Yan
1,4
Institution
1
Department of Biology
,
University of Florida
,
2
Department of Biochemistry and Molecular Pharmacology
,
New York University School of Medicine
,
3
Department of Radiation Oncology
,
Columbia University Medical Center
,
4
Center for Smell and Taste
,
University of Florida
AUTHORS
Journal (Genetics)
Site-Directed
φC31
-Mediated Integration and Cassette Exchange in
Anopheles
Vectors of Malaria
Adriana Adolfi
1,2
,
Amy Lynd
1
,
Gareth J. Lycett
1
,
Anthony A. James
2,3
1
Vector Biology Department
,
Liverpool School of Tropical Medicine
,
2
Department of Microbiology & Molecular Genetics
,
University of California
,
3
Department of Molecular Biology & Biochemistry
,
University of California
Author
Adriana Adolfi
1,2
,
Amy Lynd
1
,
Gareth J. Lycett
1
,
Anthony A. James
2,3
Institution
1
Vector Biology Department
,
Liverpool School of Tropical Medicine
,
2
Department of Microbiology & Molecular Genetics
,
University of California
,
3
Department of Molecular Biology & Biochemistry
,
University of California
AUTHORS
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